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Description
Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (HRP Conjugate)Product Specification Host Goat Antigen Rabbit IgG Immunogen Rabbit IgG Antibody Type Polyclonal antibody Isotype IgG Application ELISA, WB Reactivity Rb Purification Immunogen Affinity Concentration 1. 5 mg ml Conjugation HRP Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 20 C as supplied Dilution application dilution species ELISA 1: 1000
Product Specification
| Host | Goat |
| Antigen | Rabbit IgG |
| Immunogen | Rabbit IgG |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | ELISA, WB |
| Reactivity | Rb |
| Purification | Immunogen Affinity |
| Concentration | 1.5 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| ELISA | 1:1000-1:3000 | Rb |
| WB | 1:5000-1:10000 | Rb |
Background
Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment is a type of secondary antibody fragment that is widely utilized in various immunological assays due to its specific characteristics. Derived from immunoglobulins of goats that have been immunized against rabbit IgG, this fragment consists of the antigen-binding regions (Fab')2 of the antibody molecule, which includes both the heavy (H) and light (L) chains. The F(ab')2 fragment is obtained by enzymatic digestion of the whole IgG molecule, specifically using pepsin, which cleaves the molecule into pieces while retaining the antigen-binding sites.
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