Recombinant Carboxypeptidase B,Bovine
SKU: 30258998695

Recombinant Carboxypeptidase B,Bovine

Sale price$131.40 Regular price$146.00
Save 10%

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jul 7 - Jul 12

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Recombinant Carboxypeptidase B,BovineProduct Specification Species Bovine Synonyms Carboxypeptidase B Expression System PichiaPastoris Molecular Weight 33 kD Purity 95% by SDS PAGE & HPLC Tag His Tag Physical Appearance Lyophilized Powder Storage Buffer 20mM Tris HCl 100mM NaCl Reconstitution Carboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris HCl (pH7. 6) to make the concentration of enzyme solution 1 10mg ml. Stability & Storage 24 month, 2 to 8 C under

Product Specification


Species Bovine
Synonyms Carboxypeptidase B
Expression System PichiaPastoris
Molecular Weight

33 kD

Purity >95% by SDS-PAGE & HPLC
Tag His Tag
Physical Appearance Lyophilized Powder
Storage Buffer 20mM Tris-HCl 、100mM NaCl
Reconstitution

Carboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris-HCl (pH7.6) to make the concentration of enzyme solution 1-10mg /ml.

Stability & Storage

·24 month, 2 to 8 °C under sterile conditions
. After dissolution, store at -20℃ and avoid repeated freeze-thaw cycles.

Reference

[1] Akparov V, Sokolenko N, Timofeev V, Kuranova I. Structure of the complex of carboxypeptidase B and N-sulfamoyl-L-arginine. Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1335-40.
[2] James W. Brodrick, Michael C. Geokas, Corey Largman, Human carboxypeptidase B. II. Purification of the enzyme from pancreatic tissue and comparison with the enzymes present in pancreatic secretion, Biochimica et Biophysica Acta (BBA) - Enzymology, 1976, 468-481.

Background

Carboxypeptidase B, also known as peptidyl-L-lysine (L-arginine) hydrolase, contains one Zn atom per molecule and can selectively hydrolyze arginine and lysine at the carboxyl end of proteins or polypeptides. CPB can be used in the production of recombinant insulin and its analogues, and the quality detection of recombinant antibodies. and also used in the production of other recombinant polypeptides.

Components

20mM Tris-HCl 、100mM NaCl、Mannitol、Carboxypeptidase B

Protocol

The recommended amount of Carboxypeptidase B is 1:50~1:1000 (w/w) compared with the target protein (mass ratio), the optimal pH is 7.5-9.0, and the appropriate enzyme digestion temperature is 25℃~37℃.

Guidelines

1. For different protein samples, it is necessary to explore the optimal enzyme concentration and reaction time.

2. Reaction at 25℃~37℃, the reaction time can be appropriately extended when digestion at 25℃.

Unit Definition

The amount of enzyme that catalyzes the hydrolysis of 1 μmol of maroyl-L-arginine in 1 min is one enzyme activity unit of carboxypeptidase B.
Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 30258998695

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.3 ★★★★★
Based on 596 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
M
Verified Purchase
marcial ciprian
Waukegan, US
★★★★★ 5
Good shoes
Size: 7, Color: British Tan/Ivry, Size: 7, Color: British Tan/Ivry
Look very nice these shoes, speally to go out to dinner with the family, are really light weight, great quelith, and great softness
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 8, 2026
O
Verified Purchase
Orande Daring
Boise, US
★★★★★ 5
Nice Shoes
Size: 10.5, Color: British Tan/Ivry
Nice pair of shoes for my feet. Fits comfortably and nice cushion when walking. I would buy another pair.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 26, 2026
M
Verified Purchase
MR. H.
Lowell, US
★★★★★ 5
Super comfortable!
Size: 9, Color: Navy Blazer/Ivor
Some of the most comfortable shoes that I've ever worn/walked in! Super light on my feet. Nice casual looking and comfortable shoe for a reasonable price. I already brought 3 pair.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 21, 2026
D
Verified Purchase
deloris s.
Cuba, US
★★★★★ 5
Comfortable Shoes
Size: 12, Color: Navy Blazer/Ivor
This is the second time I purchased this style of shoes. They are extremely comfortable. They can be worn on casual and business events.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 25, 2026
L
Verified Purchase
LindyB
Lake Worth, US
★★★★★ 5
Great dressy sneaker to wear with business casual or jeans
Size: 11, Color: British Tan/Ivry
I got these for my husband to wear with jeans or business casual dress pants. They look sharp and professional and dress up jeans if you're going out. He says they fit comfortably and he can walk on them all day without his feet hurting. They are beautiful genuine leather so they don't get stinky. I got my husband's normal size and they fit to size.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 11, 2026

recommand products