E2 (Estradiol) ELISA Kit

E2 (Estradiol) ELISA KitThe E2 (Estradiol) ELISA Kit measures E2 in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with anti E2 antibody. Sample or standard completely mixes with fixed amount of biotin labeled E2 working solution and also add into each well. E2 in the sample or standard competes with biotin labeled

The E2 (Estradiol) ELISA Kit measures E2 in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with anti E2 antibody. Sample or standard completely mixes with fixed amount of biotin-labeled E2 working solution and also add into each well. E2 in the sample or standard competes with biotin-labeled E2 to bind with antibody precoated on the microplate. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of E2 in the sample. The concentration of the target substance is inversely proportional to the OD450 value.