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Ships within 48 hours · Estimated delivery Jul 8 - Jul 13
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Description
17-OHP (17-Hydroxyprogesterone) ELISA KitThe 17 OHP (17 Hydroxyprogesterone) ELISA Kit measures 17 OHP in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 17 OHP. 17 OHP in the sample or standard competes with a fixed amount of biotin labeled 17 OHP for sites on a pre coated antibody specific to 17 OHP. Free components are
The 17-OHP (17-Hydroxyprogesterone) ELISA Kit measures 17-OHP in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 17-OHP. 17-OHP in the sample or standard competes with a fixed amount of biotin-labeled 17-OHP for sites on a pre-coated antibody specific to 17-OHP. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of 17-OHP in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
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